Coding

Part:BBa_J56003

Designed by: Matt Eames   Group: iGEM06_UCSF   (2006-10-13)

Tar mutated to respond to phenylalanine instead of aspartate

Tar (aspartate-sensitive chemotaxis receptor) with mutations to respond to phenylalanine. Protein has minimal response to aspartate. Mutations include: IA2, LI21, SC27, SP32, VE41, SC43, RW73, MV76, AS101, YF149, VA196, DV249, ST255 (see reference)

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1660
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1660
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1660
    Illegal NgoMIV site found at 829
    Illegal AgeI site found at 1282
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 111


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